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rhesus monkey kidney cell line llc mk2  (ATCC)


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    Structured Review

    ATCC rhesus monkey kidney cell line llc mk2
    Rhesus Monkey Kidney Cell Line Llc Mk2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhesus+monkey+kidney+cell+line/LLC-MK2+Original/pm42215780-221-9-7
    Average 99 stars, based on 920 article reviews
    rhesus monkey kidney cell line llc mk2 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus.
    Article Snippet: .. Tulane virus was provided by L.A. Jaykus (North Carolina State University, Raleigh) and was propagated by infecting a 90% confluent monolayer of the rhesus monkey kidney cell line (LLC-MK2, ATCC CCL-7TM) at a multiplicity of infection of 0.1 (Farkas et al., 2008). .. The cells were incubated with low-serum improved minimal essential medium (Gibco Opti-MEMTM, Thermo Fischer Scientific, Grand Island, NY) supplemented with 2% v/v fetal bovine serum (Gibco FBS, Thermo Fischer Scientific) at 37 °C in a 5% CO2 atmosphere.

    Article Title: Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.
    Article Snippet: .. Hepatitis A virus (HAV pHM175 43c) was kindly provided by professor Albert Bosch (University of Barcelona), and propagated in fetal rhesus monkey kidney cell line (FRhK-4/R) (ATCC CRL-1688), as previously described (Flehmig, 1980). .. The human adenovirus 40 (AdV40) (ATCC VR-931) was cultured in PLC/PRF/5 cells (ATCC CRL-8024) according to a previous report (Grabow et al., 1992).

    Article Title: Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus
    Article Snippet: .. Tulane virus was provided by L.A. Jaykus (North Carolina State University, Raleigh) and was propagated by infecting a 90% confluent monolayer of the rhesus monkey kidney cell line (LLC-MK2, ATCC CCL-7TM) at a multiplicity of infection of 0.1 (Farkas et al., ). .. The cells were incubated with low-serum improved minimal essential medium (Gibco Opti-MEMTM, Thermo Fischer Scientific, Grand Island, NY) supplemented with 2% v/v fetal bovine serum (Gibco FBS, Thermo Fischer Scientific) at 37 °C in a 5% CO2 atmosphere.

    Infection:

    Article Title: Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus.
    Article Snippet: .. Tulane virus was provided by L.A. Jaykus (North Carolina State University, Raleigh) and was propagated by infecting a 90% confluent monolayer of the rhesus monkey kidney cell line (LLC-MK2, ATCC CCL-7TM) at a multiplicity of infection of 0.1 (Farkas et al., 2008). .. The cells were incubated with low-serum improved minimal essential medium (Gibco Opti-MEMTM, Thermo Fischer Scientific, Grand Island, NY) supplemented with 2% v/v fetal bovine serum (Gibco FBS, Thermo Fischer Scientific) at 37 °C in a 5% CO2 atmosphere.

    Article Title: Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus
    Article Snippet: .. Tulane virus was provided by L.A. Jaykus (North Carolina State University, Raleigh) and was propagated by infecting a 90% confluent monolayer of the rhesus monkey kidney cell line (LLC-MK2, ATCC CCL-7TM) at a multiplicity of infection of 0.1 (Farkas et al., ). .. The cells were incubated with low-serum improved minimal essential medium (Gibco Opti-MEMTM, Thermo Fischer Scientific, Grand Island, NY) supplemented with 2% v/v fetal bovine serum (Gibco FBS, Thermo Fischer Scientific) at 37 °C in a 5% CO2 atmosphere.



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    Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of <t>MA104,</t> Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).
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    Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of <t>MA104,</t> Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).
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    Image Search Results


    Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

    Journal: Journal of Virology

    Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

    doi: 10.1128/jvi.01883-25

    Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

    Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

    Techniques: CCK-8 Assay, Inhibition

    NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

    Journal: Journal of Virology

    Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

    doi: 10.1128/jvi.01883-25

    Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

    Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

    Techniques: Activation Assay, Expressing, Infection, Control